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JURNAL_TREUBIA
mixture of traditional medicine and for cuisine. The harvest of F. limnocharis in high volume can
threat its population. Biological data such as the age when the specimens are harvested is valuable
information to manage the harvesting system in sustainable way. We conducted the skeletochronology
technique using paraffin methods and hematoxylin staining from 69 samples (46 males, 21 females, 2
juveniles). The results showed that the age harvested male ranged from 1 to 3 years old, while the
female ranged from 2 to 3 years old. The snout-vent length (SVL) of harvested specimens ranges
between 39.84−52.37 mm for both sexes. We propose an intervention in the harvesting system by
limitation of the size for harvested specimens to at least 46 mm. In this minimum size, individuals of
F. limnocharis have reproduced several times and have contributed to the population in the wild.
Keywords: Age determination, Asian grass frog, Fejervarya limnocharis, Java, skeletochronology
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